Development of codominant simple sequence repeat, single nucleotide polymorphism and sequence characterized amplified region markers for the pea root rot pathogen, Aphanomyces euteiches.
نویسندگان
چکیده
Three kinds of genetic markers including simple sequence repeats (SSRs), single nucleotide polymorphisms (SNPs) and sequence characterized amplified regions (SCARs) were developed from Aphanomyces euteiches. Of 69 loci tested, seven SSR, two SNP and two SCAR markers were codominantly polymorphic. These codominant markers and dominant markers described herein will facilitate population genetic and evolutionary studies of this important plant pathogen.
منابع مشابه
Hierarchical Analysis of Diversity, Selfing, and Genetic Differentiation in Populations of the Oomycete Aphanomyces euteiches.
ABSTRACT Relatively little is known about the population biology of the legume pathogen Aphanomyces euteiches. A. euteiches is a soilborne pathogen causing Aphanomyces root rot of several legumes, including alfalfa, bean, lentil, and pea. Our objectives were to assess the degree of diversity, selfing, and population differentiation in A. euteiches. We contrasted populations within and among two...
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Combining plant genetic resistance with architectural traits that are unfavorable to disease development is a promising strategy for reducing epidemics. However, few studies have identified root system architecture (RSA) traits with the potential to limit root disease development. Pea is a major cultivated legume worldwide and has a wide level of natural genetic variability for plant architectu...
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Aphanomyces euteiches Drechs. causes economically important root rot diseases of several legume species when wet soil conditions prevail. This soilborne oomycete pathogen causes severe root rot damage to pea (Pisum sativum L.), alfalfa (Medicago sativa L.), snap bean (Phaseolus vulgaris L.), and red clover (Trifolium pratense L.) (12,21,22,24,25,29). A. euteiches was first described as an impor...
متن کاملRoot Rot of Pea Caused by Aphanomyces euteiches
Aphanomyces root rot, caused by the oomycete Aphanomyces euteiches, is the most important and destructive disease of pea. Since the inoculum is persistent in the soil, and no resistant cultivars or means of chemical control are available, the disease is difficult to manage. The occurrence of soil suppressiveness against the disease was previously reported, and the main aim of this study was to ...
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Oligogalacturonides (OGs) are known for their powerful ability to stimulate the plant immune system but little is known about their mode of action in pea (Pisum sativum). In the present study, we investigated the elicitor activity of two fractions of OGs, with polymerization degrees (DPs) of 2-25, in pea against Aphanomyces euteiches. One fraction was nonacetylated (OGs - Ac) whereas the second...
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 71 1 شماره
صفحات -
تاریخ انتشار 2007